Data on acute myelomonocytic leukemia published by researchers at Nagoya University

01-12-2007

Data detailed in "Establishment of a stroma-dependent human acute myelomonocytic leukemia cell line, NAMO-2, with FLT3 tandem duplication" have been presented. According to recent research from Nagoya, Japan, "We have established a stroma-dependent myelomonocytic cell line, NAMO-2, with FLT3 internal tandem duplication (FLT3/ITD). Leukemia cells from a patient with acute myelomonocytic leukemia were administered to form subcutaneous tumors in nude mice, which were maintained successively, although we failed to establish continuously growing cells from the original leukemia cell culture."

"In the cultures of cells from subcutaneous tumors, there were stroma cells that had originated from the nude mice and showed continuous growth. The leukemia cells showed continuous growth dependent on this stroma, and this cell line was named NAMO-2. Detection of FLT3/ITD by the reverse transcriptase polymerase chain reaction (PCR) and genomic PCR showed that NAMO-2 was homozygous for FLT3/ITD. Constitutive activation of FLT3 was detected by Western blotting, and the phosphorylation of Akt, MEK, and STAT5 was also observed. FLT3 kinase inhibitor AG1296 specifically inhibited cell growth," wrote A. Abe and colleagues, Nagoya University.

The researchers concluded: "NAMO-2 provides a useful tool to analyze adherence-dependent survival signaling of leukemia with FLT3/ITD and a model for the screening of FLT3 kinase inhibitors."

Abe and colleagues published their study in International Journal of Hematology (Establishment of a stroma-dependent human acute myelomonocytic leukemia cell line, NAMO-2, with FLT3 tandem duplication. International Journal of Hematology, 2006;84(4):328-36).

For additional information, contact A. Abe, Nagoya University Graduate School of Medicine, Dept. of Hematology and Oncology, Nagoya, Japan.

Publisher contact information for the International Journal of Hematology is: Carden Jennings Publ Co. Ltd., Blake Center, Ste. 200, 1224 W Main St., Charlottesville, VA 22903, USA.

Keywords: Japan, Nagoya, Acute Myelomonocytic Leukemia, Diagnosis, Diagnostics, Hematology, Monocytic Leukemia, Oncology.

This article was prepared by Hematology Week editors from staff and other reports. Copyright 2007, Hematology Week via NewsRx.com.