Study results from Shanghai Jiao Tong University, Shanghai Institute of Hematology update understanding of leukemia

02-01-2007

A new study, "Aberrant transcriptional regulation of the MLL fusion partner EEN by AML1-ETO and its implication in leukemogenesis," is now available. According to recent research from Shanghai, People's Republic of China, "The EEN (extra eleven nineteen) gene, located on chromosome 19p13, was cloned as a fusion with MLL from a patient with acute myeloid leukemia (AML) with translocation t(11;19)(q23;p13). In this study, we characterized the genomic structure of the EEN gene, including its 5' regulatory region and transcription start site (TSS)."

"We found that Sp1 could bind to the guanine-cytosine (GC)-stretch of the EEN promoter and was critical for the normal EEN expression, whereas the leukemia-associated fusion protein AML1-ETO could aberrantly transactivate the EEN gene through an AML1 binding site. Of note, overexpressed EEN showed oncogenic properties, such as transforming potential in NIH3T3 cells, stimulating cell proliferation, and increasing the activity of transcriptional factor AP-1. Retroviral transduction of EEN increased self-renewal and proliferation of murine hematopoietic progenitor cells. Moreover, Kasumi-1 and HL60-cell growth was inhibited with down-regulation of EEN by RNAi," wrote L.H. Ma and colleagues, Shanghai Jiao Tong University, Shanghai Institute of Hematology.

The researchers concluded: "These findings demonstrate that EEN might be a common target in 2 major types of AML associated with MLL or AML1 translocations, and overexpression of EEN may play an essential role in leukemogenesis."

Ma and colleagues published their study in Blood (Aberrant transcriptional regulation of the MLL fusion partner EEN by AML1-ETO and its implication in leukemogenesis. Blood, 2007;109(2):769-77).

For additional information, contact L.H. Ma, Ruijin Hospital affiliated to School of Medicine, State Key Laboratory for Medical Genomics, Shanghai Institute of Hematology, Shanghai Jiao Tong University, 197 Ruijin Road II, Shanghai 200025, China.

Publisher contact information for the journal Blood is: American Society Hematology, 1900 M Street. NW Suite 200, Washington, DC 20036, USA.

This article was prepared by Hematology Week editors from staff and other reports. Copyright 2007, Hematology Week via NewsRx.com.